Stepwise prediction of conformational discontinuous B-cell epitopes using the Mapitope algorithm
نویسندگان
چکیده
منابع مشابه
Stepwise prediction of conformational discontinuous B-cell epitopes using the Mapitope algorithm.
Mapping the epitope of an antibody is of great interest, since it contributes much to our understanding of the mechanisms of molecular recognition and provides the basis for rational vaccine design. Here we present Mapitope, a computer algorithm for epitope mapping. The algorithm input is a set of affinity isolated peptides obtained by screening phage display peptide-libraries with the antibody...
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B-cell epitopes are special regions of antigens recognized by the binding sites of immunoglobulin molecules (Van Regenmortel, 1993). These epitopes play an important role in vaccine design, disease diagnosis, and allergy research (Saha and Raghava, 2006). Although many experimental techniques have been widely used to detect B-cell epitopes, the process is both time-consuming and laborintensive....
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Discovery of discontinuous B-cell epitopes is a major challenge in vaccine design. Previous epitope prediction methods have mostly been based on protein sequences and are not very effective. Here, we present DiscoTope, a novel method for discontinuous epitope prediction that uses protein three-dimensional structural data. The method is based on amino acid statistics, spatial information, and su...
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B-cell epitopes are regions of the antigen surface which can be recognized by certain antibodies and elicit the immune response. Identification of epitopes for a given antigen chain finds vital applications in vaccine and drug research. Experimental prediction of B-cell epitopes is time-consuming and resource intensive, which may benefit from the computational approaches to identify B-cell epit...
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Antibodies have become an indispensable tool for many biotechnological and clinical applications. They bind their molecular target (antigen) by recognizing a portion of its structure (epitope) in a highly specific manner. The ability to predict epitopes from antigen sequences alone is a complex task. Despite substantial effort, limited advancement has been achieved over the last decade in the a...
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ژورنال
عنوان ژورنال: Proteins: Structure, Function, and Bioinformatics
سال: 2007
ISSN: 0887-3585
DOI: 10.1002/prot.21387